FastQCFastQC Report
Wed 3 Jul 2019
HMKYYBGXB_n01_GG030.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKYYBGXB_n01_GG030.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27836764
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG21950.026.62967746
GTCCGGT31650.016.1464251
TCGTATG38600.015.32436843
GTCGCGA5500.013.3645341
GTCCGTA26100.013.2767531
GTCCGTT51050.012.9587551
CGTATCG24050.012.66083517
CGTATGC45750.012.62341344
GTCCGAA35350.012.5751031
GTCGGTT54350.012.171931
GTCGCAT22100.012.0370071
GTTCGTT41150.011.9935151
GTTCGCG5105.657057E-1011.6674511
GTCCGCA50250.011.3539961
GTCCGGA43350.011.223361
GTCGTTT67700.011.2193651
GTCCTAT63600.011.0620641
GTCGTTA22100.010.7699541
CGCTACG14850.010.605782559
GTCCGCT62100.010.5965251
Produced by FastQC (version 0.11.5)