FastQCFastQC Report
Wed 3 Jul 2019
HMKYYBGXB_n01_GG029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKYYBGXB_n01_GG029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29813902
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGT33550.018.3620831
GTCCGTT50850.016.245121
GTCGGTT55450.013.6349661
GTCGCGA6200.012.9848821
CGTATCG33300.012.92752517
GTCCGGA44850.012.7211691
TCGACCG30000.012.71639831
GTCCGAA33750.012.5491261
GTCGTTT70450.012.5204921
GTCGGAT36650.012.5112061
GTCCATT130300.011.739211
GTCCGCA51550.011.61099151
GTCCGCT58000.011.5267591
GTCCGTA24650.011.5019091
CCCGTAT34850.011.4499591
CGTATTA14600.011.2674153
CCCGTCT70050.011.1928771
CGACCGT34600.011.12698932
GTCCCGT53550.011.1119761
CGCTACG18100.011.02186859
Produced by FastQC (version 0.11.5)