FastQCFastQC Report
Wed 3 Jul 2019
HMKYYBGXB_n01_GG028.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKYYBGXB_n01_GG028.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28140501
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG3858.185452E-1114.54501256
TCGACCG40050.011.71015331
ACGTACG26450.011.37967853
TACGTAC27350.010.87731852
GACGTAT17100.010.84762517
CGTATAG17150.010.81597919
CTCGCGT8550.010.6442951
GTCCGGT32300.010.6202121
CCCGTCT71800.010.4327541
CCCGTAT35300.010.2134431
CGACCGT46550.010.15024832
CGTATTA14200.010.10586553
ACGTATA18000.09.91634818
GTCCGCT58300.09.6664491
TACGAAA32850.09.5887556
GTCGTTT67050.09.5534881
TCCGCTA14700.09.2856632
GTCGCAT22400.09.2196331
CGTGCGA15950.09.21672538
CGTTTCG27800.09.19048331
Produced by FastQC (version 0.11.5)