FastQCFastQC Report
Wed 3 Jul 2019
HMKYYBGXB_n01_GG027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKYYBGXB_n01_GG027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24321735
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12000.014.29198948
GTCCGGT27150.012.5058631
TCGACCG29850.011.725100531
CGTATTA10950.011.5072043
GTCCGTT44000.011.2170391
CCCGTAT28000.011.0011071
GTCGCAT17350.010.8944691
CTCGCGT7450.010.8064571
GTCCGTA20550.010.5606751
GTCCGGA42350.010.4142711
CGACCGT34850.010.34420532
GTCCGCT49200.010.316081
GACGTAT15000.010.26641417
CGTGCGA11300.010.22155538
GTCGCGA5502.2730092E-810.1828441
TCCGCTA11100.010.0899452
GTCCGAA25300.09.8231241
CGTTTCG23150.09.67586731
ACGTATA15250.09.63908718
GTCGCAA13750.09.4191311
Produced by FastQC (version 0.11.5)