FastQCFastQC Report
Wed 3 Jul 2019
HMKYYBGXB_n01_GG026.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKYYBGXB_n01_GG026.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26712552
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG16300.018.89647746
CGTATTA12450.016.8678423
GTCCGGT30050.014.32762051
GACGTAT17200.013.429817
CCCGTAT34400.012.9228611
TCGACCG29350.012.64032731
ACGTACG18800.011.91457753
ACGTATA19100.011.91061218
AAGTGCG54500.011.04640539
TCCGCTA11850.010.9280822
CGTATAG20500.010.926476519
CGTTTCG22150.010.90274631
GTCCGTA21500.010.7453081
AGTGTCG59300.010.44673951
TTAACCG15100.010.430857
TACGTAC22150.010.27066552
CCCGTTT63350.010.1115531
ACCGTTA14900.010.1009710
GTCCGTT54450.010.0928821
CGTATCG26900.010.01826517
Produced by FastQC (version 0.11.5)