FastQCFastQC Report
Wed 3 Jul 2019
HMKYYBGXB_n01_GG025.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKYYBGXB_n01_GG025.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25541305
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG25400.013.09041431
GACGTAT15300.011.89502117
CGTATTA10200.011.6670473
GTCCGGT27950.011.2713091
CGTATCG25500.011.25451917
TATGCCG14300.010.76970946
GTCCGTT52100.010.4137621
GTTATCG27350.010.23737216
CGTTTCG17450.010.22911931
GTCGCAT21450.010.1176131
CGCTACT13150.010.1151311
TCGCGAA4209.26855E-69.9998952
ACGTACG17500.09.99977853
CGTATAG18600.09.97279819
CGACCGT33350.09.9699732
GTCCGTA19700.09.9502841
ACGTATA19250.09.81784818
CCCGTCT60400.09.7940761
GTCGCGA6452.5956979E-99.7684681
AAGTGCG62100.09.75089339
Produced by FastQC (version 0.11.5)