FastQCFastQC Report
Wed 3 Jul 2019
HMKYYBGXB_n01_GG024.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKYYBGXB_n01_GG024.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21008207
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG314700.14979860013755578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12250.018.00062446
CGTATTA11450.015.2843713
CCCGTAT24050.012.9537111
GACGTAT15150.012.47490717
CGTATAG16300.012.02421219
ACGTATA17450.011.43235318
AAGTGCG39350.011.38548239
ACGTACG14750.011.15234553
GTCCGGT23000.011.1100061
GTCCGTT37900.010.8983831
TTAACCG13850.010.6141367
CGTTCGA43500.010.46023244
CTCCGCT62550.010.3529391
TCGACCG24000.010.20817731
CGTTTCG17700.010.08459131
CGACCGT25700.09.94150532
GTTCGAA46250.09.83824945
AGTGTCG45800.09.7815851
GCGTTCG44100.09.76235943
CTCGTAT40550.09.6682181
Produced by FastQC (version 0.11.5)