FastQCFastQC Report
Wed 3 Jul 2019
HMKYYBGXB_n01_GG023.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKYYBGXB_n01_GG023.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24944129
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG15600.017.5007446
GTCGCGA4450.016.518281
CGTATTA10450.016.4120123
ACGTACG18900.014.07376753
GACGTAT17650.013.285748517
CCCGTAT30150.013.2349741
ACGTATA18650.013.1363118
CTCGCGT6550.012.8255351
CGTATAG18850.012.81126319
TACGTAC20750.012.48177952
GTCCGGT28450.011.5651471
TACGAAA22250.011.48284956
CTCGTAT50150.011.4466521
CCCGTCT60650.010.8500711
GTCCGTT44550.010.6855471
GACTACG23650.010.655478535
GTCCGCA44050.010.568451
TCGACCG24200.010.55770531
CTACGTA24000.010.49993951
GCTACGT24550.010.4074850
Produced by FastQC (version 0.11.5)