FastQCFastQC Report
Wed 3 Jul 2019
HMKYYBGXB_n01_GG022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKYYBGXB_n01_GG022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29045264
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19850.020.63057946
CGTATTA12500.015.4004013
CTCGCGT8350.015.0910981
ACGTACG22500.013.99966553
GTCCGGT33000.013.7890441
GACGTAT20000.013.29959117
TACGTAC24800.012.98367252
ACGTATA20250.012.61685318
TACGAAA26400.012.19663656
CCCGTAT38500.012.1828481
CCCGTCT69200.010.9763611
TCGTATG43050.010.97627443
TCGACCG34500.010.95639131
GTACGAA31250.010.86372155
CTACGTA29850.010.7871651
CGTATAG23100.010.75720819
GATCGCA30750.010.69896927
TGCTACG30700.010.60268149
GTCCGTT59450.010.5980371
CGTATGC41250.010.43690444
Produced by FastQC (version 0.11.5)