FastQCFastQC Report
Wed 3 Jul 2019
HMKYYBGXB_n01_GG021.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKYYBGXB_n01_GG021.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24041018
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA10550.014.2658843
GTCCGTA18800.013.0328691
ACGTATA15050.012.09265218
GTCCGAA28250.011.5229681
CCCGTAT28800.011.3029121
GACGTAT15800.011.07563117
CGTTTCG28100.010.83607831
GTCCGGT28750.010.8355761
TCGACCG33150.010.66343431
GTCGCAT16550.010.5747921
TATGCCG12100.010.12430946
CTCGCGT7251.9463187E-109.6558811
GTCGCGA6908.112693E-109.6383871
CGTATAG18600.09.59647819
GTCCGCT52950.09.58521651
CTCGTAT46750.09.58359051
GCCCGAT33300.09.4601531
GTCCGTT48850.09.3865621
GACTACG20950.09.35567235
CGTATCG36200.09.28150317
Produced by FastQC (version 0.11.5)