FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18109178
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1171200.6467438776072553No Hit
GGGGGGCGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG268100.14804647676443403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG44200.059.6233865
GTATAGC40200.057.9818576
GTATAGG89000.055.2890626
TATAAGG89700.054.0771147
CGTATAC23800.053.967595
CGTATAA69600.053.402215
GGTATAG90250.052.972135
CTATAGG49550.052.9032176
GGGTATA77700.052.429524
CGCTATA41550.051.8021774
GCGTATA58450.051.61394
GCTATAA76450.051.592955
TATAGGC57450.050.5018427
CCGTATA39200.050.3543474
TATACGG44100.050.3145267
CATATAG71600.049.7598085
TATAGCA59050.049.7261477
ACGTATA58300.049.5855834
CCTATAG34950.049.568065
TCGTATA56700.049.318254
Produced by FastQC (version 0.11.5)