FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14791433
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG91800.064.5838245
GGTATAG163550.062.5077745
CTATAGG92700.062.3333366
GTATAGC76150.061.586846
GTATAGG176050.061.2503666
CCTATAG62200.060.9946755
GGATAGG82150.059.2188876
CGGTATA109250.059.1375434
GGGTATA157400.058.9242634
CGTATAA137450.058.9212045
CGCTATA91100.058.7411084
GGTATAA177400.058.081425
GCTATAA147650.057.90865
TATAGGC110000.057.4934657
TATAGCG89350.057.306367
CGTATAC48250.057.303725
ATAGGGC43450.057.1901938
TATAAGG175300.057.1400687
GCTATAG112200.056.990015
CCGTATA71550.056.7904854
Produced by FastQC (version 0.11.5)