FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13940430
Sequences flagged as poor quality0
Sequence length76
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG164750.062.9018145
GTATAGG172050.061.9213186
CGTATAG82700.061.829385
CTATAGG83600.060.3683326
GGGTATA149400.059.923914
GTATAGC66850.059.735766
CGGTATA103450.059.644724
GGTATAA161700.059.1101235
TATAGCG73900.058.2046437
CCTATAG55000.058.1612475
GGATAGG82500.058.1188246
CGCTATA73000.057.8195424
CGTATAA118450.056.080435
TATAGGC98000.055.9262737
GCGCTAC9800.055.3548665
GCGATAG44650.055.260895
TATAGTG236550.055.1277287
TATAAGG171600.055.0880627
TCTACCC22750.055.074667
GGGATAG90650.055.0556535
Produced by FastQC (version 0.11.5)