FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16295341
Sequences flagged as poor quality0
Sequence length76
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG100150.063.2178655
GTATAGG201350.062.1929176
GGTATAG194200.062.121835
TATAGCG93800.059.8485687
CGGTATA120800.059.5673644
CTATAGG105700.059.1369136
CCTATAG60700.059.0999835
GTATAGC79900.059.090466
GGGTATA179250.058.7705234
CGCTATA90650.057.835734
GGTATAA196950.057.1672445
TATAAGG191100.056.9209987
GGATAGG100900.056.781876
CGTATAA146550.056.623665
TATAGTG274350.056.0413327
ATAGGGG106050.056.0045628
TATAGGC118400.055.7200937
CCTATAA109350.055.4666565
CGTATAC45100.055.408185
GCTATAG130950.055.030455