FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10006918
Sequences flagged as poor quality0
Sequence length76
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG57750.062.1200145
GGTATAG114850.061.4659465
GTATAGG118350.061.3042686
CGGTATA70200.059.877764
CTATAGG63300.059.769786
CCTATAG38200.059.4622045
GGATAGG60450.058.7664456
GGGTATA100350.058.41944
TATAGCG58100.058.1314137
GGTATAA105600.057.8019185
CGCTATA47250.057.7025954
GTATAGC48150.057.1328546
CGTATAC25650.056.8994875
ATAGGGG60850.056.3095138
CGTATAA77100.056.1986545
TATAGGC73300.056.1039627
GCTATAG78550.055.3394785
TTATAGC72450.055.119716
TATAAGG123700.055.002987
CCGTATA42450.054.8281564
Produced by FastQC (version 0.11.5)