FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14873222
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG85250.063.387645
GGTATAG173250.062.2602845
GTATAGG187400.062.0787736
CTATAGG91950.061.2048766
GGGTATA167350.060.4194
CCTATAG57250.059.9715465
GTATAGC74650.058.885936
CGCTATA81650.058.852654
CGGTATA107700.058.331144
GGTATAA184400.058.286855
TATAGCG89150.057.2384457
CGTATAA129750.057.1038255
CGTATAC41750.057.087675
GGATAGG93400.056.994646
TATAAGG177300.056.7519537
TATAGGC100850.056.567037
TATACGG89300.056.4368447
GCTATAG116350.056.3107345
ATAGGGG89200.055.8723378
CCTATAA100950.055.366915
Produced by FastQC (version 0.11.5)