FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9321741
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG122350.062.5588535
CGTATAG58600.060.738965
GTATAGG125500.060.2078176
GGTATAA115500.059.6936655
CGGTATA68150.059.108984
GGGTATA111900.058.7054184
CTATAGG62450.058.3954856
TATAGCG57550.058.319977
GTATAGC51400.058.080446
GCGATAG31500.057.5523645
CCTATAG37150.057.278125
CGCTATA47300.056.9735874
TATAAGG126350.056.8112567
GCTATAG74300.056.383155
ATAGGGG68350.055.4541328
GGATAGG63100.055.020726
CGTATAA77950.054.9103625
CTATAGC39850.054.714666
TATAGGC70950.054.556537
TATAGTG166700.054.5230757
Produced by FastQC (version 0.11.5)