FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12341427
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG158900.062.860645
GTATAGG170050.062.7317056
CGTATAG70300.062.0811655
CTATAGG82150.060.3685846
GGGTATA151450.060.3604774
CGGTATA86950.059.6925774
GGATAGG85400.059.0547566
TATAGCG69750.058.5565387
TATAAGG169150.058.4100577
CCTATAG45900.058.330745
CGCTATA54350.058.01954
ATAGGGG97800.057.93718
GGTATAA154350.057.865915
GTATAGC56450.057.659086
CGTATAA97300.056.7960365
TATAGGC91550.056.578597
CGTATAC30150.056.41545
ATAGGTG96350.056.229998
GCTATAG94900.056.130285
GGGATAG91200.056.0665865
Produced by FastQC (version 0.11.5)