FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15500321
Sequences flagged as poor quality0
Sequence length76
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG87400.063.029115
GGTATAG177050.062.5839355
GTATAGG183400.062.1345186
CTATAGG97000.059.8580476
CGGTATA104350.059.6668054
GGGTATA164000.059.1986584
TATAGCG90050.058.6480267
GGTATAA173950.058.146195
GTATAGC74050.058.1336986
CGCTATA77150.056.9320834
GGATAGG94300.056.48736
CCTATAG62900.056.364635
ATAGGGG96950.056.2067768
TATAGGC109850.055.8827067
TATAGTG268250.055.449437
CGTATAA126450.055.4383135
GCTATAG118050.055.143565
ATAGGTG100400.054.867978
CGTATAC44450.054.485665
TATAAGG200450.054.4225167
Produced by FastQC (version 0.11.5)