FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16380047
Sequences flagged as poor quality0
Sequence length76
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG186600.062.213575
CGTATAG93250.062.15315
GTATAGG203150.061.727876
CGGTATA112850.060.1040234
GGGTATA175800.059.0278744
CTATAGG102800.058.796376
GGATAGG106750.058.3256236
CCTATAG61250.058.2263265
TATAGCG88300.057.9479377
GTATAGC75850.057.769696
TATAGGC114550.056.7677847
GGTATAA196250.056.6041875
TATAAGG204350.056.484247
GGGATAG110200.056.4678765
CGCTATA77000.056.0887454
CGTATAC44150.055.7283175
ATAGGTG115750.055.4535838
ATAGGGG109950.055.450348
TATAGTG281850.055.133597
GCGATAG50700.054.948575
Produced by FastQC (version 0.11.5)