FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11755371
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG74200.063.5827945
GGTATAG147050.061.9292565
GTATAGG156950.060.587356
CTATAGG77600.060.255736
GGGTATA136750.059.5300144
TATAGCG67050.059.349267
GTATAGC58850.059.1739046
GGTATAA138650.058.310365
GGATAGG78100.057.9430126
CGCTATA60050.057.6417164
CCTATAG46600.057.3049665
TATAAGG153850.057.235677
CGTATAA94400.057.2067835
CGGTATA86700.057.20114
GCTATAG97450.056.7092485
ATAGGGG84050.056.4228748
GCGATAG38050.055.8325735
CCGTATA51050.055.8061754
CGTATAC33650.055.2284775
TATAGTG223400.055.0518847
Produced by FastQC (version 0.11.5)