FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12936165
Sequences flagged as poor quality0
Sequence length76
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG72050.063.1961865
GTATAGG152850.062.0513766
GGTATAG142800.060.8059585
CTATAGG83550.060.1527566
CGGTATA92200.059.4060944
GTATAGC58750.058.975946
GGGTATA135200.058.81384
TATAGCG74700.058.61177
CCTATAG49750.058.600255
GGATAGG80400.058.374116
GGTATAA153500.057.912585
CGTATAA111900.056.923145
TATAAGG154950.056.263887
TATAGGC89350.056.169717
ATAGGGG85500.056.038088
TATAGTG211150.055.576357
GCTATAG97350.055.5084235
TATAGGT188100.055.372197
CGCTATA60650.055.1663974
GCTATAA119000.055.0856325
Produced by FastQC (version 0.11.5)