FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14506784
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG83950.062.3678675
GGTATAG169650.062.240275
GTATAGG181050.061.8394626
CTATAGG94500.059.8123326
CGGTATA101250.059.074294
GTATAGC68650.058.7812426
GGGTATA155850.058.3198854
CGCTATA65050.058.1607364
ATAGGGG97800.057.7227448
CCTATAG55950.057.67415
TATAAGG174250.057.4036457
GGATAGG95750.057.3135456
TATAGCG83900.057.1907127
GGTATAA170450.056.835425
CGTATAA117450.056.2003065
TATAGGC104500.055.6962477
TATACGG80000.055.122717
GGGATAG98050.054.9339755
TATAGTG249450.054.802297
CGTATAC36700.054.7388735
Produced by FastQC (version 0.11.5)