FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14051582
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG157150.063.115255
GTATAGG168200.062.2772676
CGTATAG79950.062.073555
CTATAGG90350.061.319926
CGTATAC33300.059.697065
CGGTATA97550.058.9825254
GGGTATA145950.058.9422344
CCTATAG50750.058.6180955
GGTATAA162500.058.28055
GGATAGG86450.058.2160456
GTATAGC65950.058.215776
TATAGCG86550.058.067917
CGCTATA65200.057.489794
CGTATAA111700.057.4325525
ATAGGGG93900.057.361688
TATAGGC100100.057.1653027
TATACGG83750.055.6632087
GGGATAG92500.055.6575
TATAAGG170800.055.489357
TATAGGT214750.055.4434177
Produced by FastQC (version 0.11.5)