FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9723971
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG98810.10161486495589096No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG61250.064.511216
CGTATAG67400.064.492475
GTATAGC52900.062.3883256
GTATAGG126700.062.179356
GGTATAA115300.062.1349335
CGTATAC32450.062.1233865
TTATAGC69300.062.0677456
GGATAGG48050.062.057396
CGGTATA81500.062.009314
GGTATAG115550.061.879355
CGTATAA96300.061.3106735
TATAAGG108750.061.0177767
GCTATAG68300.060.9780585
GGGTATA102150.060.7459954
TATAGGG144950.060.6766477
CCTATAG43800.060.5678865
AGTATAG91150.060.0903855
CTATAGC45650.059.8766636
CCTATAC29300.059.8435555
TATAGCG74800.059.703037
Produced by FastQC (version 0.11.5)