FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12710322
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG169950.062.4599235
GTATAGG170750.061.5113756
CGTATAG74200.061.1767275
CTATAGG83700.059.9198766
CGGTATA97400.059.828374
GGGTATA151750.058.304264
GCGATAG38650.058.134745
GGATAGG81900.057.9890676
GGTATAA156800.057.6761975
ATAGGGC38750.057.44288
GTATAGC61300.056.6371466
TATAAGG169600.056.3567667
TATAGCG72350.056.3556637
CGCTATA63150.056.308284
CGTATAA104950.055.9910285
TATAGTG230150.055.368137
CCTATAG53800.055.3601765
ATAGGGG91500.054.8884548
ATAGGTG92650.054.887118
CGTATAC36350.054.7845465