FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13865536
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCC12700.063.9349177
CGTATAG89550.061.8684435
GTATAGG129350.061.3663756
CGGTATA116900.061.2257654
CTATAGC60050.060.789026
CTATAGG67950.060.7780046
GGTATAG139600.060.771655
CCTATAC48200.060.4855775
GGTATAA174250.060.175945
CGTATAA166350.059.057285
TATAGCG71250.058.9454467
CGTATAC49850.058.694175
ATAGGGC39950.058.345828
AGTATAG116000.058.2308585
GTATAGC79850.058.119586
CGCTATA101300.058.078274
GGGTATA132300.057.9348264
GCTACCC5200.057.2096677
CGGATAG34350.057.159715
TATAGCA115450.057.144087
Produced by FastQC (version 0.11.5)