FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13215500
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG81400.063.9786345
GTATAGG156800.062.9670646
GGTATAG147200.062.865165
CTATAGG86750.061.2433136
CGGTATA96250.060.3620154
CGTATAC37900.059.9324345
GTATAGC66450.059.6221776
CGCTATA74300.059.258174
GGTATAA154850.058.7197725
GGGTATA136000.058.520494
TATAGCG87400.058.3851627
CGTATAA120100.057.5838055
CCTATAG57500.057.4593285
GGATAGG77450.057.0746
GCTATAG100600.056.847385
TATAAGG165300.056.7226147
GCTATAA133850.056.6365135
TTATAGC97750.056.2491346
CCTATAC38400.056.144335
TATAGGC99050.056.04097
Produced by FastQC (version 0.11.5)