FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13220057
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG88750.064.3583455
GGTATAG144450.064.231085
CTATAGG92550.062.812536
GTATAGG163350.062.6271636
CGTATAC39000.062.1004265
CGGTATA94500.061.257154
GGTATAA147300.061.0399865
CCTATAG59100.060.700115
GTATAGC68000.060.5787936
GGGTATA143100.060.36134
CGTATAA118450.060.0992365
TATAGCG107250.059.9792867
CCTATAA102550.059.144725
TATAAGG156750.058.521117
GCTATAG103500.058.3651355
GGATAGG76600.058.163796
CGCTATA77900.058.136634
TTATAGC100650.057.8271186
GCTATAA132300.057.6964235
AGTATAG128950.057.5125585
Produced by FastQC (version 0.11.5)