FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5596465
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG36250.063.3345645
GGTATAG72900.062.3629385
GGTATAA66950.060.6390385
CTATAGG40300.060.0960586
TATAGCG37000.059.6860127
GTATAGG77150.059.335796
CGTATAC18350.059.1249165
GGGTATA72400.059.02314
CCTATAG25300.057.9613465
CGGTATA44050.057.363584
CGCTATA34500.056.910024
CGGATAG17800.056.8228235
GTATAGC32500.056.7508286
GGATAGG37100.056.6950956
TATAAGG73700.056.4149067
TATACGG34100.055.935447
GCTATAG50550.055.3877565
GCGATAG19700.055.073215
TATAGGT84150.054.691097
CGTATAA50600.054.6413655