FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13284136
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG160000.063.106226
GGTATAG155600.062.2141085
CGTATAG84750.061.8612145
CTATAGG90050.061.446126
GGGTATA148050.060.4461484
CCTATAG57850.060.256285
GGTATAA159400.059.8746725
CGGTATA98450.058.6562844
TATAGCG86150.058.337197
GGATAGG79900.058.2574126
CGCTATA74600.058.1740344
GTATAGC66900.057.9642146
CGTATAC36100.057.6840975
GCTATAG105400.057.5113535
CGTATAA116700.057.460715
TATAAGG168100.056.879967
CCTATAA90200.056.6490555
TATAGGT193150.056.6422587
TATAGGC99500.056.5951587
ATAGGGG91850.056.35588
Produced by FastQC (version 0.11.5)