FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12866175
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG97450.061.9885335
GGTATAA151500.061.634755
GGTATAG168850.061.4991235
CTATAGG101700.061.428476
CGTATAA118850.060.839215
GTATAGG184350.060.239226
GTATAGC78950.060.2004976
GGGTATA148500.059.2977644
ACTATAG106800.058.6917085
TTATAGC106600.058.1451876
GCTATAG113200.057.9705285
ATTATAG198700.057.9319885
CCTATAG66250.057.8998035
TATAGCA101450.057.8540047
TATAGCG102700.057.558797
TATAAGG195600.057.436597
CGTATAC42600.057.0988855
CTATAGT115150.057.0192266
CCTATAA100050.056.809555
CGCTATA79850.056.804694
Produced by FastQC (version 0.11.5)