FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12248219
Sequences flagged as poor quality0
Sequence length76
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG131040.10698698316873662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG67000.063.7283555
GGTATAG121300.062.321895
GTATAGG130850.062.320356
CTATAGG66900.061.6787266
GGTATAA130850.060.4213185
GGGTATA119750.059.8552324
CGTATAC30350.059.2723465
CGGTATA82800.059.2182354
CGGATAG32700.059.0797925
GGATAGG66350.058.3921366
CCTATAG48450.058.150015
GTATAGC55700.057.6184846
CGTATAA104150.057.49615
TATAGCG77300.057.228887
TATAGGC83200.056.9564637
CCGTATA55000.056.124644
GCTATAG84900.055.898445
TATAAGG133050.055.7658967
CGCTATA61100.055.7338834
GTCTACC17650.055.5214776
Produced by FastQC (version 0.11.5)