FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13510777
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG186500.064.273636
CGTATAG94050.064.043225
CTATAGG98900.063.7689976
GGTATAG176200.063.5616575
GGTATAA165400.062.3161935
CGTATAC36050.062.2305765
GTATAGC71100.061.875226
CCTATAG56850.061.255315
CGTATAA114100.060.856525
GGGTATA169800.060.289174
CGGTATA100400.060.027544
GCTATAG106600.059.78665
TATAAGG175950.059.6737177
ATAGGGG140350.059.4241188
AGTATAG139050.058.8722085
TATAGCG93850.058.54867
CCTATAA91450.058.477765
GGATAGG91950.058.4262166
GTCTACC23000.058.280336
TATAGTG201200.058.116527
Produced by FastQC (version 0.11.5)