FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11079763
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG68950.062.2304955
GGTATAG138800.061.9782685
GGTATAA124750.059.9810685
GTATAGG146500.059.8196376
CGCTATA65600.059.43314
CTATAGG75750.059.3700686
GTATAGC59750.059.043176
TATAGCG73850.058.291027
CGTATAA87600.058.290565
GGGTATA129500.058.240434
CGGTATA82800.058.2037054
TATAAGG142050.057.7761577
GCGATAG38750.057.4423835
CGTATAC36550.057.3570065
GCTATAA107550.055.3855135
CCTATAG46850.055.2801445
GCTATAG93150.055.0805365
TTATAGC92250.054.783266
TATAGGC88950.054.3762367
GTATAAG135900.054.3387956
Produced by FastQC (version 0.11.5)