FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14243238
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG151750.1065417849508658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG92200.063.6577155
GGTATAG169750.063.4198765
GTATAGG187600.062.9822926
CTATAGG99500.062.926876
GGTATAA162350.061.869775
CGTATAC36700.061.414195
CGGTATA107250.060.3376664
CGGATAG44100.060.31485
CGTATAA120450.060.00155
AGTATAG143850.059.802715
GGGTATA166800.059.442824
GTATAGC73100.059.3681036
GCTATAG114000.059.2210665
GGATAGG92250.059.1843836
ATAGGGG115400.059.0788468
TATAGCG97950.058.9560557
TATAAGG184750.058.422327
CGCTATA72950.058.1948284
CCGTATA59350.058.0850524
CCTATAG56950.058.0132455
Produced by FastQC (version 0.11.5)