FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8415103
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG107200.066.5696646
CGTATAG71300.066.463485
CTATAGG65600.066.156386
CGTATAC36900.065.824415
CCTATAC33100.065.239575
GTATAGC56150.064.824246
GGTATAA106950.064.532725
GGTATAG96800.064.393565
CGTATAA100600.063.979035
CTATAGT64500.063.6490866
GCTATAG71150.063.3078465
TATAGGG125350.063.1570977
CGGTATA76350.062.9841884
AGTATAG77700.062.8808335
GTATACG53550.062.808416
GTCTACC16200.062.652286
GGGTATA97450.062.5992934
TTATAGC64700.062.5868346
ACTATAG63750.062.5861935
CATATAG79050.062.559635
Produced by FastQC (version 0.11.5)