FastQCFastQC Report
Thu 11 Jul 2019
HMKWVBGXB_n01_Lex21TFs-1-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKWVBGXB_n01_Lex21TFs-1-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5569
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160.2873047225713773No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAA150.002109743270.000015
TATAGTG150.002109743270.000017
TATAGTC150.002109743270.000017
TATAGCA150.002109743270.000017
GTATATG252.4859528E-670.06
TATATTG207.2550894E-569.999997
TTTATAG207.2550894E-569.999995
ATATAGT207.2550894E-569.999996
ATATAAA252.1890731E-455.9999968
TATATAA200.00659486852.4999967
TATAAAA305.3856114E-446.6666689
ACACGTC350.001150903240.036
CACACGT350.001150903240.035
ACGTCTG400.002218550834.99999638
CACGTCT400.002218550834.99999637
AAGAGCA400.002218550834.99999630
GAGCACA400.002218550834.99999632
AGCACAC400.002218550834.99999633
GCACACG400.002218550834.99999634
AGAGCAC450.003952789631.11111331
Produced by FastQC (version 0.11.5)