FastQCFastQC Report
Sat 13 Jul 2019
HMKNCBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKNCBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30102401
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2295890.7626933147292803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA149300.034.2609816
ACACGTC153150.034.14892213
ACGTCTG152300.033.6774415
CACGTCT160050.032.2639114
TATACGG13550.031.823727
CACACGT173700.031.02983912
GCACACG176050.030.83347311
GTATACG10500.030.471856
CGTATAA12850.028.958735
CGTATAG9400.028.118015
GCGTATA11200.027.9486524
GAACTCC178100.027.3284521
CTATACG7850.026.138626
TTATACG12350.025.9072426
GTCACCG41400.025.79551129
TATAGCG10400.025.746967
CGGAAGA233350.025.5767784
CCGTATA9300.025.4309354
TATACGA13750.025.290997
GACGTTA27500.025.1508159
Produced by FastQC (version 0.11.5)