FastQCFastQC Report
Sat 13 Jul 2019
HMKNCBGXB_n01_ba166.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKNCBGXB_n01_ba166.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30833023
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA14450.020.104443
CCCGTAT32350.017.3111531
GACGTAT33350.014.58764617
CGCCTAT14250.014.2460161
ACGTATA33950.014.123651518
ACCGTTA21250.013.50643910
TTAACCG22650.013.4444147
CGTATAG36950.013.16632219
CTCGTTT116200.012.6810811
ACGATAC69600.012.37057860
CGCGTTT11150.011.9285881
CCGTATT38850.011.8922272
CTCGTAT46200.011.8185161
CATACGA73950.011.69022657
CCGTTAA25100.011.57388111
GACTACG44000.011.53398635
ATACGAT76050.011.4134458
CCCGCAT54400.011.3881891
GCTACGA25000.011.33989744
CGATACT77700.011.21611561
Produced by FastQC (version 0.11.5)