FastQCFastQC Report
Sat 13 Jul 2019
HMKNCBGXB_n01_ba165.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKNCBGXB_n01_ba165.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35224047
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT40400.015.5078721
CGTATTA18250.013.4250823
CTCGTTT108850.012.3154681
CTCCGAT95900.011.8251581
CTCGTAT54650.010.9518241
ACCGTTA23000.010.50045110
CGCCTAT18550.010.3776571
CCCGTTT72200.010.3742591
CCCGAAT62100.010.3143061
CTCCTAT97650.010.1078181
CCCGATT34950.09.9144491
CTCCCGT87400.09.8515411
CTCAAAT231000.09.6517931
CTCCGCT114900.09.5955771
CGCATAT21050.09.4777071
TTAACCG25550.09.4525957
CCCCGCT98900.09.449211
CGCGTTT15750.09.3336011
CTCCGGT98650.09.2957571
CGCTAAT17000.09.2649731
Produced by FastQC (version 0.11.5)