FastQCFastQC Report
Sat 13 Jul 2019
HMKNCBGXB_n01_ba164.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKNCBGXB_n01_ba164.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45832819
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA19600.014.6434113
CTCGTTT145600.011.3465341
CCCGTAT53000.011.2928311
CGCCTAT19300.09.6117211
CTCCGAT128150.09.3955551
CCCGAAT78350.09.2919511
CCCGATT43850.09.2591481
ACCGTTA26950.09.22111810
CTCCTAT106550.09.1978681
TCGTTTT165400.09.1629432
CGTTTTG147350.08.8364393
CTCGTAT71500.08.7625311
CTCAAAT276200.08.7312841
CCGGCAT86300.08.7198751
CGCTAGT20650.08.6443581
CCCGTTT86950.08.574181
CGCCAAT62550.08.5054811
TTAACCG30100.08.4888057
CGCTATT23150.08.4668061
CTCCGGT131150.08.3799991
Produced by FastQC (version 0.11.5)