FastQCFastQC Report
Sat 13 Jul 2019
HMKNCBGXB_n01_ba163.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKNCBGXB_n01_ba163.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38785622
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC27250.010.78888244
TCGTATG28850.09.826591543
CTCGTAT28400.09.6125842
ATGCCGT31350.09.26625547
TCTCGTA29100.09.26107541
TGCCGTC33850.08.27170448
GCCGTCT34550.07.80021149
TATGCCG38750.07.76766446
CCGTCTT40750.06.613430550
CGTCTTC50400.06.180493451
ATCTCGT50250.05.85068840
CATCTCG56750.05.30391139
Produced by FastQC (version 0.11.5)