FastQCFastQC Report
Sat 13 Jul 2019
HMKNCBGXB_n01_ba162.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKNCBGXB_n01_ba162.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35765807
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2080330.5816533092626709TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG274750.062.08869643
CTCGTAT264000.061.87262342
CGTATGC277450.061.56016544
ATGCCGT277350.061.40569347
TCTCGTA258400.061.31725341
GATCTCG250750.061.23381839
CGATCTC251200.061.08233338
CACGATC252900.060.6578936
GCCGTCT281100.060.34994549
TGCCGTC282500.060.13758548
TATGCCG284550.059.91343746
ACGATCT256550.059.71304337
CCGTCTT286150.059.22372450
TCACGAT269250.059.2233135
ATCTCGT262850.058.5481540
CATCACG291200.058.49714333
ATCACGA282500.058.42786434
CGTCTTC291150.058.2908151
CGTCTGA306550.056.9953916
ACGTCTG319050.054.9927515
Produced by FastQC (version 0.11.5)