FastQCFastQC Report
Sat 13 Jul 2019
HMKNCBGXB_n01_ba161.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKNCBGXB_n01_ba161.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37479597
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1771640.47269451696612425TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT219150.062.02996442
TCGTATG233050.061.7994343
CGTATGC235250.061.72734544
TCTCGTA219050.061.56296541
AATCTCG220850.060.93442539
ATGCCGT236000.060.908347
GCCGTCT236800.059.5644549
TGCCGTC239750.059.51766648
TATGCCG245600.058.88378546
CCGTCTT242600.058.35680850
CGTCTTC245050.057.57340251
ATCTCGT235200.057.1869440
CGTCTGA272300.056.51645716
ACGTCTG280600.054.9195715
ACACGTC286200.053.90633413
CACGTCT303800.050.79470414
GTATGCC288700.050.49309545
GCACACG320350.048.24881711
GATCGGA329550.047.44341
ATCGGAA331100.047.274162
Produced by FastQC (version 0.11.5)