FastQCFastQC Report
Sat 13 Jul 2019
HMKNCBGXB_n01_ba160.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKNCBGXB_n01_ba160.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37209980
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC730510.19632098700402417TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT115200.051.2842847
CGTATGC117450.050.98721344
CTCGTAT111000.050.5446142
TCGTATG113650.050.505543
TCTCGTA111450.049.83806641
TGCCGTC118800.049.25883548
GCCGTCT115300.048.23462349
TATGCCG126850.047.4020346
CCGTCTT120750.046.202550
CGTCTTC124650.044.39191451
ATCTCGT127150.044.12466440
TCGCGTA9050.043.31454541
CATCTCG138050.040.6914339
CGCGTAT10450.038.18148842
GTATGCC172500.035.22288545
ATCGCGT11300.034.999740
CATCGCG14550.030.30901539
GATCATC231400.026.95309336
CCGTCGT10200.026.4213450
GCCGTCG10150.026.20666949
Produced by FastQC (version 0.11.5)