FastQCFastQC Report
Sat 13 Jul 2019
HMKNCBGXB_n01_ba159.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKNCBGXB_n01_ba159.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34670872
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2797110.8067607875567709TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG331650.062.99204343
CTCGTAT329750.062.81368342
CGTATGC333900.062.72479244
TCTCGTA330300.062.64550841
CGCCAAT338200.062.3308933
TATCTCG333900.061.86526539
TATGCCG338800.061.7556446
ATGCCGT338750.061.6924347
ATCTCGT340250.060.83412640
ACGCCAA348150.060.4891832
TGCCGTC347450.060.0368748
GCCGTCT345950.059.8520449
GTCACGC354200.059.49550229
CCGTCTT351000.059.06071550
AGTCACG365400.057.71978428
CACGCCA364900.057.7125531
TCACGCC365300.057.67809330
CGTCTTC361350.057.41749251
CGTCTGA376700.057.14068616
ACACGTC387100.055.9403813
Produced by FastQC (version 0.11.5)