FastQCFastQC Report
Sat 13 Jul 2019
HMKNCBGXB_n01_ba158.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKNCBGXB_n01_ba158.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32020849
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC398340.12440019938259601TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC74450.054.25065244
TCGTATG72350.053.5032943
CTCGTAT61100.050.8669442
TCTCGTA61250.050.3423741
ATGCCGT80550.050.18573847
TATGCCG82400.049.78107546
TGCCGTC86000.046.35420248
GATCTCG65350.046.2199139
CGTCTGA99250.045.03238316
GCCGTCT87350.043.47410649
CCGTCTT88950.043.36102350
ACACGTC105950.042.77973613
ATCTCGT71050.042.56116540
GATCGGA108950.042.2453881
ACGTCTG107950.041.5327815
CGTCTTC93050.040.77338451
ATCGGAA113550.040.4724122
GTCGTAT21400.036.9622642
CACGTCT122450.036.9292914
GTATGCC117800.035.6533145
Produced by FastQC (version 0.11.5)