FastQCFastQC Report
Sat 13 Jul 2019
HMKNCBGXB_n01_ba157.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKNCBGXB_n01_ba157.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36602137
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2805540.7664962294414668TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT332150.062.6653342
TCGTATG348250.062.5119343
TCTCGTA329250.062.22867641
CGTATGC353700.061.72682644
AATCTCG324500.061.53249739
ATGCCGT354000.061.46688547
TGCCGTC357300.060.81102448
TATGCCG359800.060.6511446
GCCGTCT358000.060.3499549
CCGTCTT359850.060.06886750
ATCTCGT335750.059.6896440
CGTCTTC364450.059.20505551
CGTCTGA388900.058.35381716
ACGTCTG399850.056.77329315
ACACGTC404000.056.23356613
GTATGCC403500.054.2646345
CACGTCT421900.053.90581514
GCACACG450050.050.6138511
ATCGGAA459750.050.580942
GATCGGA461750.050.2859951
Produced by FastQC (version 0.11.5)