FastQCFastQC Report
Sat 13 Jul 2019
HMKNCBGXB_n01_ba156.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKNCBGXB_n01_ba156.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40920941
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC11474792.8041363955926624TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA1405050.066.4098734
TCTCGTA1271500.066.3576641
CTCGTAT1289000.066.3392242
ACCGATG1416100.066.3093632
TCGTATG1292500.066.3085143
CACCGAT1420450.066.22455631
CGTATGC1328300.066.21344
ATGCCGT1321050.066.1233447
CCGATGT1417200.066.1146433
TATCTCG1292250.065.9367439
TATGCCG1339300.065.8730146
TGCCGTC1309450.065.84309448
GTCACCG1428750.065.8422929
GCCGTCT1272100.065.7320649
CGTCTGA1458700.065.6063616
CCGTCTT1286500.065.5621950
ATCTCGT1294300.065.49428640
TCACCGA1440200.065.37958530
CGTCTTC1271100.065.2909151
ACGTCTG1471450.065.0783215
Produced by FastQC (version 0.11.5)